Protocols
BASIC RESEARCH
Many of the protocols the Jepsen Laboratory uses for basic research were assembled for Current Protocols in Mouse Biology.
(Clicking on links below will open the protocol .pdf file in a new window)General bone preparation protocols
NanoCT protocols. These protocols are designed for a GE nanoTom S system. If you have a different microCT or nanoCT system, you will need to adjust the protocol.
- Protocol for scanning adult mouse femurs
- Protocol for scanning adult mouse lumbar vertebrae
- Protocol for scanning developing mouse femurs (Will be uploaded soon, please check back)
- Protocol for scanning developing mouse lumbar vertebrae (Will be uploaded soon, please check back)
Mechanical testing protocols. These protocols were written for use with a custom testing frame used on an MTS mini-bionix system.
- Mouse 4 point bending protocol
- Mouse vertebral compression testing (Will be uploaded soon, please check back)
Other protocols
TRANSLATIONAL RESEARCH
General bone preparation protocols
NanoCT protocols. The nanoCT system allows us to scan both cadaver and dry bone at high resolution.
pQCT protocols. We have scanned a wide range of bones including live individuals, cadavers, anatomical collections, and archeological collections.
- Protocol for scanning cadaver bones
- Protocol for scanning dry bones (Will be uploaded soon, please check back)
- Protocol for analyzing pQCT images of cadaver bones
Mechanical testing protocols. These protocols are designed for use with custom fixtures on an Instron testing frame.
Other protocols
- Basic Fuchsin Staining and Embedding
- MMAI-MMAIV stock solutions(.pdf, will open in a new window)
NANOCT SCANNING
These protocols are designed for a GE nanoTomS system. If you have a different microCT or nanoCT system, you will need to adjust the protocol.
Mouse nanoCT protocols.
- Protocol for scanning adult mouse femurs
- Protocol for scanning adult mouse lumbar vertebrae
- Protocol for scanning developing mouse femurs (Will be uploaded soon, please check back)
- Protocol for scanning developing mouse lumbar vertebrae (Will be uploaded soon, please check back)
Human nanoCT protocols.
MECHANICAL TESTING
Mouse mechanical testing protocols. These protocols were written for use with a custom testing frame used on an MTS mini-bionix system.
- Mouse 4 point bending protocol
- Mouse vertebral compression testing (Will be uploaded soon, please check back)
Human mechanical testing protocols. These protocols are designed for use with custom fixtures on an Instron testing frame.